Page 4 - BRIP ePoster Book

The role of the oncogenic TBX3 in the transformation of mesenchymal stem cells into a subset of sarcomas

Authors: Damerell, V. , Willmer, T. , du Toit, M. , Swartz, S. , Lennard, K. , Mulder, N. , Govender D. , Pepper, MS. and Prince, S. 1
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1 Department of Human Biology, Department of Integrative Biomedical Sciences, Department of Pathology, Faculty of Health Sciences, University of Cape Town, South Africa, Department of Immunology, Faculty of Health
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Sciences, University of Pretoria, South Africa, Biomedical Research and Innovation Platform, SAMRC, Tygerberg 7505, South Africa
Empty #1 Empty #2 Synovial Adjacent
1 Liposarcoma Chondrosarcoma Fibrosarcoma sarcoma normal tissue
TBX3 is upregulated
in sarcoma tissues TBX3
Immunohistochemistry
TBX3 #1 TBX3 #2 TBX3 FC relative to normal by stage Logrank Test P-Value: 0.0591
Fold change
TBX3 p=0.02 TBX3

3D-Spheroid invasion: 48 hours I-III IV
Tumour stage
4 2 PCR Array Kaplan-Meier curve
TBX3 promotes TBX3 promotes
MSC invasion MSC proliferation
MMP2 70 kDa
1.2 1.0 1.8 1.2 1.3
MMP9 70 kDa
1.1 1.0 1.3 1.5 1.1
β-actin 43 kDa

Western Blot: Invasion markers TBX3 95 kDa
Cyclin A 54 kDa MTT Assay: Growth curve

3 Cyclin B1 54 kDa
TBX3 promotes CDK2 34 kDa
MSC migration β-actin 43 kDa

Migration Assay Western Blot: Cell cycle markers