Page 7 - BRIP ePoster Book

THE EFFECT OF PINOCEMBRIN ON IMPAIRED MITOCHONDRIAL BIOENERGETICS ASSOCIATED WITH
DOXORUBICIN ADMINISTRATION IN AN MCF-7 CELL MODEL
Nonhlakanipho F. Sangweni, Lawrence Mabasa, Derick van Vuuren, Barbara Huisamen, Rabia Johnson
Background Results Table 1: Normalized mitochondrial flux ratios

Oxidative phosphorylation
During chemotherapy, distinct
bioenergetics dysfunction in cancer A B Basal respiration
mitochondrial subpopulations may be 400 Oligo FCCP Rot + Ant A 200
OCR (pmol/min/ug protein) 200 OCR (pmol/min/ug protein) 100 aaa
reversed by the co-administrative use ***
of Doxorubicin (Dox) with 300 150 ***
cardioprotective agents.

100
50
0 0 10 20 30 40 50 60 70 80 0 Mitochondrial membrane potential
Control Pin Dox
Time (min) Dox + Pin

AIM C 150 ATP linked respiration D 250 Spare respiratory capacity
***
To assess the degree of impaired mitochondrial function associated with the 200 ** ** G
combined use of Dox plus Pinocembrin (Pin), using an MCF-7 breast cancer 100 aa 150 4
cell model OCR (pmol/min/ug protein) OCR (pmol/min/ug protein) 100 aaa 3 ***

Methodology 50 aaa 50 Mitochondrial potential (AU) 2
1 0 0 aaa
MCF-7 Cells 1 aaa
Control Pin Dox Dox + Pin Control Pin Dox Dox + Pin 0

Pin
E 40 ATP turnover F 400 Maximal respiration Control Conclusion Dox Dox + Pin
OCR (pmol/min/ug protein) 30 aa OCR (pmol/min/ug protein) 300 *** aaa aaa Although Pin, as an adjunct to Dox, influenced the respiratory
***

200
20
parameters of the cancer cells, this effect was not sufficient to
100
ameliorate the cells mitochondrial function as determined by the
10
Group 1 Group 2 0 0 reduced mitochondrial flux ratios and potential (ΔΨm).
2 Control Pin Dox Control Pin Dox Acknowledgements
Control Dox Dox + Pin Dox + Pin
Pin Dox + Pin Reenen Barry, Bio-Pharm New Zealand,
SAMRC Research Capacity Development PhD Internship Program,
aa p < 0.01, aaa p < 0.001 compared to control, and ** p < 0.01, ***p < 0.001 compared to Dox. Results expressed as Biomedical Research and Innovation Platform
mean ± SEM